苯达松敏感致死恢复系的辐射诱变和筛选

为了选育对苯达松敏感的水稻恢复系的突变体。利用350 Gy的60Coγ-射线对粳稻恢复系C418和籼稻恢复系浙恢7954进行辐射诱变,M0代舒城育种基地种植并单株收种,收回的M1代分别取6000粒种子于光照培养室中培养,在3叶期用浓度为400.0 mg/L的苯达松溶液涂抹叶片进行筛选。60Coγ-射线诱变对水稻种子有很大的生理损伤,M1代植株育性发生变化的有70%左右;M2代的苗期出现了一定数量的白化苗和黄化苗。用浓度为400.0 mg/L苯达松溶液涂抹叶片筛选出4株C418敏感突变体,突变率为0.07%,筛选出3株浙恢7954敏感突变体,突变率为0.05%。筛选出的突变株基本保持起始亲本的农艺性状。辐射诱变与杂交转育方法相比,具有方法简单、周期短的优点。

Radiation Induced-mutation and Screening of Bentazon Susceptible Lethality Restorer

The study aimed to breeding mutant of rice restorer, which was sensitive to bentazon.Both Japonica restorer C418 and indica restorer Zhehui 7954 were mutated by 350 Gy 60 Coγ-ray irradiation.M0 generation was planted in Shucheng breeding base and the single-plant was harvested for planting.6 000 seeds were chosen from the harvested M1 and cultured in plant growth chamber.The screening was conducted by painting 400.0 mg/L bentazon solution on the leaves in their three-leaf period.60 Coγ-ray irradiation made severe physiological damage to rice seeds and the fertility-changed plants rate was about 70% in M1 generation.The certain amount whitening and etiolated seedlings appeared in the seedling stage of M2 generation.The sensitive mutants of 4 plants of C418 and 3 plants of Zhehui 7954 were screened out by painting leaves with bentazon solution at 400.0 mg/L and their mutation rates were 0.07% and 0.05% resp.The agronomic characters of initial parents were basically kept in the screened mutated plants.Compared with hybrid transferring breeding,the radiation induced-mutation had advantages of simple method and short cycle.