松材中两种重要线虫比较的研究

松材线虫(Bursaphelenchus xylophilus)和拟松材线虫(B.mucronatus)被进一步证明是两个不同的种。除表现致病力差异外,形态上具有明显的不同特征,特别是前者交合伞卵圆形,后者平截形;生理学试验表明二者不能交配产生后代;生物化学方面的差异是:酯酶凝胶电泳中松材线虫酯酶带3条,迁移率分别为0.42、0.45、0.49;而拟松材线虫只1条酯酶带,迁移率为0.65。二者脂肪酸总量、不饱和度、短链和长链脂肪酸含量都有明显的差异。

STUDIES ON CONTRAST BETWEEN THE TWO IMPORTANT SPECIES OF NEMATODES IN PINE WOOD

Bursaphelenchus xylophilus and B. mucronatus were further confirmed to be two different species. The pathogenicity and the bursa forms of the twospecies evidently differ from each other, the bursa of the former species isegg-shaped, while that of the latter species is flatmouth-shaped. The physio-logical tests indicated that the two species couldn't mate with each other.Biochemical experiments showed that the former species esterase had three iso-enzyme bands, and its relative electrophoretic mobilities (Rf) were 0. 42, 0. 45and 0.49 respectively, and that the latter species'esterase only had oneisoenzyme band and the Rf was 0.65. On the other hand, there were obviousdifferences between the two species about the distribution of fatty acids.