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| 不同耐药水平大肠杆菌acrA和marA基因转录水平的比较 | |
| 引用本文： | 柳巨雄,吉淑娟,杨斌,张海旺,王玮,胡仲明,曾凡勤,邓旭明,韩春田. 不同耐药水平大肠杆菌acrA和marA基因转录水平的比较[J]. 中国兽医学报, 2006, 26(3): 326-328,332 |
| 作者姓名： | 柳巨雄 吉淑娟 杨斌 张海旺 王玮 胡仲明 曾凡勤 邓旭明 韩春田 |
| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062 2. 河北沽源县畜牧局,河北,沽源,076550 3. 石家庄市畜牧水产局动物医院,河北,石家庄,050000 4. 河北北方学院,河北,张家口,075131 5. 军事医学科学院,军事兽医研究所,吉林,长春,130062 |
| 摘 要： | 为阐明大肠杆菌耐药菌株药物敏感性变化与acrA和marA的mRNA水平之间的关系。用定量RT—PCR方法比较了多重耐药菌株、单药耐药菌株以及质控株ATCC25922的actA和marA的mRNA水平。结果。actA mRNA水平。多重耐药菌株SEMR是单药耐药菌株SEICI、SEICH和质控株ATCC25922的16倍；／natA mRNA水平。SEMR是SEICH的4倍、SEICI和ATCC25922的8倍；SEICI和SEICH的actA mRNA、marA mRNA水平与ATCC25922的无明显差异；同一菌株actA mRNA和marA mRNA水平的变化保持了较为稳定的一致性。结论：大肠杆菌多重耐药菌株的actA mRNA和marA mRNA水平与其耐药水平存在相关性。 |
| 关 键 词： | 大肠杆菌 acrA基因 marA基因 转录水平 |
| 文章编号： | 1005-4545（2006）03-0326-03 |
| 收稿时间： | 2004-06-29 |
| 修稿时间： | 2004-06-29 |
Comparison of acrA mRNA and marA mRNA Levels in Escherichia coli with Multidrug Resistance |
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| LIU Ju-xiong,JI Shu-juan,YANG bin,ZHANG Hai-wang,WANG Wei,HU Zhong-ming,ZENG Fan-qin,DENG Xu-ming,HAN Chun-tian. Comparison of acrA mRNA and marA mRNA Levels in Escherichia coli with Multidrug Resistance[J]. Chinese Journal of Veterinary Science, 2006, 26(3): 326-328,332 | |
| Authors: | LIU Ju-xiong JI Shu-juan YANG bin ZHANG Hai-wang WANG Wei HU Zhong-ming ZENG Fan-qin DENG Xu-ming HAN Chun-tian |
| Affiliation: | 1. College of Animal Science and Veterinary Medicine ,Jilin University, Changchun ,Jilin 130062 ,China ;2. Animal Husbandry Bureau of Guyuan County ,Guyuan , Hebei 076550, China; 3. Shijiazhuang Animal Husbandry Bureau ,Shijiazhuang 050000, China ; 4. Hebei North College ,Zhangjiakou ,Hebei 075131 ,China ; 4. Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062,China |
| Abstract: | The purpose of this experiment is to detect acrA mRNA and mRNA levels in Escherichia coli with multidrug resistance, clarify the correlation between the resistance level of Escherichia coli resistance stratins and the levels of acrA mRN and marA mRNA. The method is to compare the levels of acrA mRNA and marA mARA of SEICI, SEICH and ATCC25922 with quantitative RT-PCR. The results were as follows: the acrA mRNA level of SEMR is 16 times as high as that of SEICH, SEICH and ATCC25922; the marA mRNA level of SEMR is 4 times as high as that of SEICH and 8 timess as high as that of SEICI and ATCC25922; the acrA mRNA and marA mRNA levels of SEICI and SEICH were not significant statistically compared with that of ATCC25922; the acrA mRNA and marA mRNA levels in the same strains were coincident .The results showed that there was a correlation between the acrA mRNA and marA mRNA levels and resistance level of SEMR. |
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