| 4种抗凝剂对刺参体腔细胞的抗凝效果 |
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| 引用本文： | 张峰,宋志东,刘洪伟,宫静,王海峰.4种抗凝剂对刺参体腔细胞的抗凝效果[J].大连水产学院学报,2006,21(1):87-89. |
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| 作者姓名： | 张峰 宋志东 刘洪伟 宫静 王海峰 |
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| 作者单位： | 大连水产学院,生命科学与技术学院,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023 |
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| 摘 要： | 研究了不同浓度的柠檬酸钠、肝素钠、乙二胺四乙酸二钠(EDTA-Na2)和草酸钠对刺参Stichopus japonicus体腔细胞的抗凝效果。结果表明,3.8 mg/mL的柠檬酸钠、1.0~1.5 mg/mL的ED-TA-Na2、0.2~0.6 mg/mL的肝素钠、1.0~1.5 mg/mL的草酸钠均能较好地保持体腔细胞的形态活性,适合用作刺参体腔细胞的抗凝剂。4种抗凝剂中EDTA-Na2的效果最好。
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| 关 键 词： | 刺参 柠檬酸钠 肝素钠 乙二胺四乙酸二钠 草酸钠 体腔细胞 抗凝剂 |
| 文章编号： | 1000-9957（2006）01-0087-03 |
| 收稿时间： | 03 4 2005 12:00AM |
| 修稿时间： | 2005-03-04 |
Effects of four anticoagulants on anticoagulation of coelomocytes in sea cucumber Stichopus japonicus |
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| ZHANG Feng,SONG Zhi-dong,LIU Hong-wei,GONG jing,WANG Hai-feng.Effects of four anticoagulants on anticoagulation of coelomocytes in sea cucumber Stichopus japonicus[J].Journal of Dalian Fisheries University,2006,21(1):87-89. |
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| Authors: | ZHANG Feng SONG Zhi-dong LIU Hong-wei GONG jing WANG Hai-feng |
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| Institution: | School of Life Science and Technology, Dalian Fisheries Univ. , Dalian 116023, China |
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| Abstract: | Effects of different levels of sodium citrate,EDTA-Na_(2),HEPES,and sodium oxalate on anticoagulation of coelomocytes were studied in sea cumcumber Stichopus japonicus.The results showed that the coelomocytes were morphologically kept activity at 1.0-1.5 mg/mL EDTA-Na_(2),3.8 mg/mL sodium citrate,(0.2)-0.6 mg/mL HEPES,and 1.0-1.5 mg/mL sodium oxalate,EDTA-Na_(2) being the best anticoagulant. |
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| Keywords: | Stichopus japonicus sodium citrate HEPES EDTA-Na_2 sodium oxalate coelomocyte anticoagulant |
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