乙基多杀菌素在香蕉果实、花瓣和土壤中的残留及消解动态分析

为评估乙基多杀菌素在香蕉上的安全性使用,本研究采用超高效液相色谱-串联质谱(UPLC-MS/MS)法,检测香蕉植株和土壤中乙基多杀菌素的残留及降解动态。结果表明,乙基多杀菌素在香蕉果实、花瓣和土壤中的检出限(LOD)均为5μg/kg,定量限(LOQ)均为17μg/kg。当乙基多杀菌素在果实、花瓣和土壤中的添加浓度在0.01~0.20 mg/kg时,添加回收率分别为89.6%~95.2%、76.5%~89.8%和75.8%~86.5%,相对标准偏差分别为5.03%~7.10%、5.16%~7.24%和2.26%~5.83%。乙基多杀菌素在香蕉果实、花瓣和土壤中的半衰期分别为5.6、4.5和6.5 d,属于易消解型农药。UPLC-MS/MS法具有灵敏度高、速度快等特点,可利用此法检测香蕉中乙基多杀菌素残留及消解动态。

Analysis of Insecticide Residue and Digestion Dynamics of Spinetoram in Banana Fruits,Petals and Soil

In order to evaluate the safety of spinetoram on banana, the residue and degradation dynamics of spinetoram in banana were detected by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that the limits of detection (LOD) of spinetoram in banana fruit, petal and soil were both 5 μg/kg. And the limits of quantification ( LOQ) were both validated calculated as 17 μg/kg. At the spiked levels of 0.01-0.20 mg/kg, the average recoveries of acetochlor in fruit, petal and soil were 89.6%-95.2%, 76.5%-89.8% and 75.8%-86.5%, and the relative standard deviations was 5.03%-7.10%, 5.16%-7.24% and 2.26%-5.83%, respectively. The half-lives of acetochlor in fruit, petal and soil was 5.6 d, 4.5 d and 6.5 d. Taken together, the findings reveal that spinetoram belongs to the easily degradable pesticide type. The present study suggests that UPLC-MS / MS could be applied to detect and analyze spinetoram residues and digestion dynamics in banana.