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联苯肼酯与炔螨特混配对朱砂叶螨的联合毒力作用
引用本文:王 雪,梁 晓,卢芙萍,卢 辉,伍春玲,陈 青. 联苯肼酯与炔螨特混配对朱砂叶螨的联合毒力作用[J]. 热带作物学报, 2017, 38(5): 932-938. DOI: 10.3969/j.issn.1000-2561.2017.05.024
作者姓名:王 雪  梁 晓  卢芙萍  卢 辉  伍春玲  陈 青
作者单位:1. 海南大学环境与植物保护学院,海南海口 570228;中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带作物病虫害生物防治工程技术研究中心 海南省热带农业有害生物监测与控制重点实验室 海南海口571101;2. 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带作物病虫害生物防治工程技术研究中心 海南省热带农业有害生物监测与控制重点实验室 海南海口571101
基金项目:国家木薯产业技术体系害虫防控岗位科学家专项(CARS-12-hncq),科技部星火计划重点项目(2015GA800002),中央级公益性科研院所基本科研业务费专项(2016hzs1J021)
摘    要:为了科学合理使用2008年在中国获得登记的新型酰基乙腈联苯肼类杀螨剂联苯肼酯,延长其使用寿命,本研究采用生物测定法和紫外分光光度法针对性地开展了联苯肼酯与炔螨特混配对朱砂叶螨的联合毒力作用评价。结果表明,43%联苯肼酯悬浮剂和73%炔螨特乳油按有效成分215∶73混配对朱砂叶螨的共毒系数为163.0,共毒系数大于120,表现出良好的增效作用,经其20.00、14.29、11.11、9.09和7.69 mg/L的浓度处理24 h后,朱砂叶螨的谷胱甘肽S-转移酶(GST)活性显著降低,分别为3.371、3.645、3.932、4.045和4.089 mmol/(mg Pro·min),显著低于相同浓度联苯肼酯单剂[7.250、6.818、6.714、6.441、5.811 mmol/(mg Pro·min)]和炔螨特单剂[10.130、9.789、9.142、8.932、8.441 mmol/(mg Pro·min)],说明联苯肼酯与炔螨特混配对朱砂叶螨GST具有显著的联合抑制作用;经联苯肼酯与炔螨特混配液处理24 h后,朱砂叶螨的多功能氧化酶(MFO)、羧酸酯酶(Car E)和乙酰胆碱酯酶(Ach E)活性与联苯肼酯和炔螨特单剂处理的结果无显著差异。本研究结果为合理混配使用联苯肼酯与炔螨特防治朱砂叶螨的发生与危害提供了理论依据。

关 键 词:朱砂叶螨  联苯肼酯  炔螨特  联合毒力  谷胱甘肽S-转移酶(GST)  多功能氧化酶(MFO)  羧酸酯酶(CarE)  乙酰胆碱酯酶(AchE)

The Joint Toxicity Effect of Bifenazate and Propargite Against Tetranychus cinnabarinus
WANG Xue,LIANG Xiao,LU Fuping,LU Hui,WU Chunling and CHEN Qing. The Joint Toxicity Effect of Bifenazate and Propargite Against Tetranychus cinnabarinus[J]. Chinese Journal of Tropical Crops, 2017, 38(5): 932-938. DOI: 10.3969/j.issn.1000-2561.2017.05.024
Authors:WANG Xue  LIANG Xiao  LU Fuping  LU Hui  WU Chunling  CHEN Qing
Abstract:Bifenazate is a new type of acyl acetonitrile hydrazide acaricide which was registered in China in 2008.In order to use bifenazate scientifically and reasonably,and prolong the serv ice life,the joint toxicity effect of bifenazate and propargite against Tetranychus cinnabarinus was evaluated in this study using bioassay and UV Spectrophotometry.The results showed that,with the effective components ratio of 215:73 for 43% bifenazate suspension concentrate (SC) and 73% propargite emulsifiable concentrate (EC),the cotoxicity coefficient (CTC) was 163.0,which showed synergistic toxicity to T.cinnabarinus as the CTC over 120.While T.cinnabarinus was treated by the mixture of bifenazate and propargite with the concentration of 20.00,14.29,11.11,9.09 and 7.69 mg/L for 24 h,the activity of glutathione S-transferase (GST) in T.cinnabarinus was 3.371,3.645,3.932,4.045 and 4.089 mmol/(mgPro·min),which was significantly (p<0.05) lower than the activity of GST in T.cinnabarinus being treated by bifenazate [7.250,6.818,6.714,6.441 and 5.811 mmol/(mgPro·min)] and propargite [10.130,9.789,9.142,8.932 and 8.441 mmol/(mgPro·min)] at the same concentrations.The data indicated that the mixture of bifenazate and propargite showed a joint inhibition effect on the activity of GST in T.cinnabarinus.Moreover,the activities of mixed-function oxidase (MFO),carboxylesterase (CarE) and acetylcholinesterase (AchE) in T.cinnabarinus were not significantly different compared with those being treated by bifenazate and propargite individually.This study would provide a theoretical basis for reasonable application of bifenazate and propargite in T.cirmabarinus control.
Keywords:Tetranychus cinnabarinus  bifenazate  propargite  joint toxicity effect  glutathione S-transferase (GST)  mixed-function oxidase (MFO)  carboxylesterase (CarE)  acetylcholinesterase (AchE)
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