五指毛桃抗炎活性成分的分离及鉴定

以对5-LOX的抑制活性为抗炎活性评价指标,在活性跟踪下从五指毛桃中分离到6个化合物,经波谱数据分析后被鉴定为:羽扇豆醇棕榈酸酯(1)、β-香树脂醇乙酸酯(2)、补骨脂素(3)、芹菜素(4)、壬二酸(5)和胡萝卜苷(6),其中,化合物5、1对5-LOX表现出强的抑制活性,半数抑制浓度(IC50)分别为13.74μg/m L和13.96μg/m L;化合物1为首次从五指毛桃中分离得到。本研究结果可为五指毛桃的抗炎作用研究及其临床应用提供理论参考。

Isolation and Identification of Chemical Constituents with Anti-inflammatory Activity from the Roots of Ficus hirta Vahl.

To investigate the anti-inflammatory activity of the roots of Ficus hirta Vahl.5-lipoxygenase (5-LOX) inhibitory activity was selected as the evaluation index.Six compounds were separated from this material by 5-zlipoxygenase inhibitory activity-guided isolation.Their structures were identified as lupeol palmitate (1),β-amyrin acetate (2),psoralen (3),apigenin (4),azelaic acid (5),daucosterol (6) on the basis of NMR spectroscopic analyses.Compound 5 and 1 exhibited strong in vitro inhibitory activity on 5-LOX with IC50 values 13.74 μg/mL and 13.96 μg/mL,respectively.Compound 1 was reported for the first time from F.hirta roots.The results may provide theoretical supports for the anti-inflammatory effect and clinical application of F.hirta roots.