王氏唇柱苣苔的离体培养及遗传稳定性

以王氏唇柱苣苔(Chirita wangiana)叶片为外植体进行组培离体快繁研究,并利用流式细胞术对组培苗进行遗传稳定性分析。结果表明:最佳初代诱导培养基为MS添加0.5 mg/L 6-BA+0.1 mg/L NAA;最适继代培养基为MS添加0.5 mg/L 6-BA+0.05 mg/L NAA;最适生根培养基为1/2 MS培养基添加0.5 mg/L IBA和15 g/L蔗糖,所有生根培养基获得的组培苗移栽驯化成活率达到92%以上。组织学切片检测表明,王氏唇柱苣苔叶片为外植体所形成芽体均为器官发生方式形成。流式细胞术检测表明,组培苗倍性没有变化,基因组大小与母本相比仅发生了1.86%的减少;染色体数目为2n=36,跟母本一致,植株形态特征上也无变异。研究结果可为王氏唇柱苣苔的园艺应用快速提供大量遗传稳定的种苗。

In vitro Rapid Propagation of Chirita wangiana and Genetic Stability

In vitro rapid propagation of Chirita wangiana using the leaf section as the explant was studied,and the genetic stability of the regenerated plantlets was analyzed by flow cytometry and chromosome number.The results showed that the most optimal bud induction medium was MS medium supplemented with 0.5 mg/L 6-BA and 0.1 mg/L NAA;the suitable subculture medium was MS medium with 0.5 mg/L 6-BA and 0.05 mg/L NAA;1/2 MS medium supplemented with 15 g/L sucrose and 0.5 mg/L IBA was suitable for rooting.Histological investigation revealed that the buds and plantlets regenerated through organogenesis.Ploidy investigation by flow cytometry showed that there was no ploidy variation among the regenerated plantlets,though there was only a 1.86％ decrease in the genome size.Both the mother plant and in vitro regenerated plantlets had the same chromosome numbers (2n=36) and unchanged morphological characteristics.The results could be used for large scale rapid propagation of genetic stable seedlings of C.wangiana in horticultural application.