| 不同光质条件下铁皮石斛多糖含量与磷酸烯醇式丙酮酸羧化酶基因表达变化 |
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| 引用本文： | 林小苹,赖钟雄. 不同光质条件下铁皮石斛多糖含量与磷酸烯醇式丙酮酸羧化酶基因表达变化[J]. 热带作物学报, 2017, 38(5): 838-842. DOI: 10.3969/j.issn.1000-2561.2017.05.009 |
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| 作者姓名： | 林小苹 赖钟雄 |
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| 作者单位： | 1. 漳州城市职业学院,福建漳州 363000;福建农林大学园艺植物生物工程研究所,福建福州 350002;2. 福建农林大学园艺植物生物工程研究所,福建福州,350002 |
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| 基金项目： | 漳州市自然基金项目(zz2013J12),福建省农业科技平台建设项目(2015N20002-1) |
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| 摘 要： | 以铁皮石斛试管苗为材料,研究不同光质下铁皮石斛磷酸烯醇式丙酮酸羧化酶(PEPC)和多糖积累的差异。结果表明:红、蓝和绿3种光质下多糖含量、PEPC活性和PEPC基因表达量变化趋势基本一致,表现为红光蓝光绿光;在白光下则出现显著差异,呈现出基因表达量低、酶活性高、多糖含量高的特征,显示出光质对光合代谢调控的复杂性。
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| 关 键 词： | 光质 铁皮石斛 多糖含量 PEPC活性 PEPC基因表达量 |
Effects of Light Quality on Expression of PEPC and Polysaccharide Accumulation in Dendrobium officinale |
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| LIN Xiaoping and LAI Zhongxiong. Effects of Light Quality on Expression of PEPC and Polysaccharide Accumulation in Dendrobium officinale[J]. Chinese Journal of Tropical Crops, 2017, 38(5): 838-842. DOI: 10.3969/j.issn.1000-2561.2017.05.009 |
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| Authors: | LIN Xiaoping and LAI Zhongxiong |
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| Abstract: | The effects of light quality on polysaccharide accumulation,PEPC activities and PEPC gene expression were investigated in Dendrobium officinale.The results showed that the change trends of PEPC activities,polysaccharide contents and PEPC expression levels were consistent in the descending order red light,blue light,reen light,whereas they appeared significantly different in the white light,showing lower PEPC gene expression-level,higher PEPC activities,and more polysaccharide accumulation,which implied the complexity of the regulation of different light quality on the photosynthetic metabolism. |
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| Keywords: | Light quality Dendrobium officinale polysaccharide content PEPC activity PEPC gene expression |
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