红掌愈伤组织诱导和芽的分化

 对影响红掌愈伤组织诱导及芽分化的几个因素进行了研究。同一成熟度的外植体, 其叶柄愈伤组织诱导率、芽分化率、分化时间均明显优于叶片。不同放置方式和光照时间对叶片愈伤组织的诱导亦有影响, 叶背向下放置, 光照时间24 h/ d 和10 h/ d 愈伤组织诱导率较高, 分别为100 %、97 %。光照时间对叶柄愈伤组织诱导无显著影响, 但光照24 h/ d 和10 h/ d 较无光照处理的明显促进芽的分化。叶柄培养以N6 , KC 和1/ 2 MS 培养基为佳。叶片培养则以P , N6 和1/ 2 MS 为好。以未展叶叶柄为外植体, 从接种到丛芽分化共49 d , 较已有报道提前11～31 d。

The Callus Induction of Anthurium andraeanum Linden and Bud Differentiation

The factors that influence the callus induction and bud differentiation of Anthurium andraeanum Linden were studied.Petiole showed significantly better results than blade in callus induction,bud differentiation and duration for bud formation.The callus induction on blade was influenced by the way of placement on medium and period for lighting,and the highest percentage(100% and 97%)for callus induction were recorded under the condition that the downside of blade were against the medium and with 24h/d and 10h/d light.Differnet light treatments did not affect the callus induction of petiole significantly.However,the 24h/d and 10h/d light treatment obviously improved the bud differentiation compared to the treatment with no light.Promising results among several mediums tested were recorded:N 6,KC and 1/2 MS for petiole and P,N 6 and 1/2 MS for blade, respectively. The time from explant to bud differentiation were 49d,which was 11-31d earlier than time previously reported.