小麦雄性不育系和保持系花药ATP酶细胞色素氧化酶细胞化学定位

本文运用电子显微镜细胞化学标记技术，对K型雄性不育系和其保持系小麦花药各个组织中的细胞色素氧化酶和ATP酶活性进行了定位，相对定量研究。结果表明，药壁四层组织和药隔细胞中两种酶的活性分布在花粉粒败育前，不育系与其保持系之间几乎没有差别，均在细胞核、核仁、质膜与胞间连丝中有ATP酶的活性，线粒体内嵴上有细胞

Ultracytochemical Localization of ATPase and Cytochrome Oxidase in the Anther from K-77(2) A and 77(2)B

Ultracytochemical localization of cytochrome oxidase and ATPase in anther from cytoplasm male sterile K-77 (2) A] and its maintainer 77 (2) B] was studied with electron microscopic cytochemical technique. The results indicated that the activity distribution of ATPase and cytochrome oxidase in epidermis, endotheium, middle-layer, tapetum and vascular cells from K-77 (2)A was similar to that from 77 (2)B, and in both cases the reaction product was mainly distributed on nucleus, nucleolus, plasma membranes and plasmodesmata, and the two enzymes' activity in the vascular cells was stronges than in other cells. During uninucleate pollen stage, the activity of ATPase was observed in nucleus and nucleolus, but the activity of cytochrome oxidase was not observed in mitochondria from both K-77 (2)A and 77 (2)B. At early bincleate stage, plasma membranes and mitochondria in pollens of K-77 (2)A and 77 (2)B showed weak activity of ATPase and cytochrome oxidase. In the course of pollen growth and its intine formation, two enzymes' activity in the pollens from 77 (2) B increased gradually. When the intine had been formed, it revealed remarkable ATPase activty, and the pollens were full of mitochondria with developed cristae where cytochrome oxidase activity was observed. ATPase activity on plasma membranes of the pollen from K-77 (2) A increased slightly, and the intine development was abnormal and there was weak ATPase activity in the intine. It was found that there is no direct correlation between pollen abortion and the two enzymes' activity in the epidermis, endothecium, middle-layer, tapetum and vascular cells, but that there is close correlation between the two enzymes' actcvity in the pollen and pollen abortion.