| mRNA差异显示技术评述 |
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| 引用本文： | 刘中成,赵锦,刘孟军. mRNA差异显示技术评述[J]. 分子植物育种, 2004, 2(6): 895-900 |
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| 作者姓名： | 刘中成 赵锦 刘孟军 |
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| 作者单位： | 河北农业大学生命科学学院,保定,071001;河北农业大学中国枣研究中心,保定,071001 |
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| 摘 要： | 真核生物mRNA差异显示技术自1992年建立以来，经过不断的改进与完善已经成为分子生物学领域的重要技术平台之一，为基因的差异表达及表达基因的克隆研究提供了强有力的工具。本文主要对mRNA差异显示技术的原理、优缺点及其近年来的改进与发展进行了介绍和评述。
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| 关 键 词： | mRNA差异显示 |
An Introduction to mRNA Differential Display Technique |
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| Liu Zhongcheng Zhao Jin Liu Mengjun College of Life Science,Agricultural University of Hebei,Baoding, Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding. An Introduction to mRNA Differential Display Technique[J]. Molecular Plant Breeding, 2004, 2(6): 895-900 |
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| Authors: | Liu Zhongcheng Zhao Jin Liu Mengjun College of Life Science Agricultural University of Hebei Baoding Research Center of Chinese Jujube Agricultural University of Hebei Baoding |
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| Affiliation: | Liu Zhongcheng1 Zhao Jin1 Liu Mengjun2*1 College of Life Science,Agricultural University of Hebei,Baoding,0710012 Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding,071001 |
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| Abstract: | Since established in 1992, Eukaryotic mRNA differential display has been modified a lot and has become one of the most important molecular biotechniques, which provided a powerful method to identify and clone differentially expressed genes. In this paper, the principle, advantages, disadvantages as well as recent development of this technique were briefly introduced. |
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| Keywords: | mRNA differential display |
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