| 禽白血病病毒分离鉴定 |
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| 引用本文： | 范书才,李虹,肖睿.禽白血病病毒分离鉴定[J].中国兽药杂志,2006,40(9):31-33. |
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| 作者姓名： | 范书才 李虹 肖睿 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 摘 要： | 使用 SPF鸡胚成纤维细胞从蛋用种鸡的病料中分离到一株病毒.经禽白血病病毒（ALV） p-27抗原ELISA检测、病毒培养、反转录-聚合酶链式反应（RT-PCR）鉴定、群特异性抗血清中和试验和动物回归试验等证明,该株病毒属于禽白血病病毒.
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| 关 键 词： | 禽白血病病毒 分离 鉴定 |
| 文章编号： | 1002-1280（2006）09-0031-03 |
| 收稿时间： | 2006-03-03 |
| 修稿时间： | 2006年3月3日 |
Isolation and Identification of Avian Leukosis Virus |
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| FAN Shu-cai,LI Hong,XIAO Rui.Isolation and Identification of Avian Leukosis Virus[J].Chinese Journal of Veterinary Drug,2006,40(9):31-33. |
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| Authors: | FAN Shu-cai LI Hong XIAO Rui |
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| Institution: | China Institute of Veterinary Drug Control, Beijing 100081 ;China |
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| Abstract: | A strain of virus was isolated from laying breeding chicken using primary chicken embryo fibroblasts(CEFs) freshly prepared from embryos(9~10 days old) of the line 0 leghorn chicken strain held as a specific pathogen-free(SPF) flock and identified to be the avian leukosis virus(ALV) by the assays such as detection of the isolate using ELISA with ALV p-27 Ag,virus propagation in CEFs,detection of viral genome by RT-PCR,neutralization of virus by subgroup-specific antiserum and infection of 1-day-old SPF chicken inoculated using the isolate. |
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| Keywords: | Avian leukosis virus isolation identification |
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