Maintenance and growth ofHelminthosporium solani

We have found that by using water agar as an isolation medium, V-8 juice agar as a growth substrate, and sterilized rye grains as a storage medium we can: 1) produce copious amounts ofHelminthosporium solani conidia that were successfully used as inoculum and: 2) the sterilized rye grains could be used to maintain viable cultures of the five isolates ofH. solani used in this study for 16 months. All of the isolates were pathogenic on tubers of the potato cultivars Katahdin and Superior.