Glandular trichomes: Enzymic browning assays for improved selection of resistance to the green peach aphid |
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| Authors: | James D. Ryan Peter Gregory Ward M. Tingey |
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| Affiliation: | 1. Department of Plant Breeding and Biometry, Cornell University, 14853, Ithaca, New York, USA
2. Department of Entomology, Cornell University, 14853, Ithaca, New York, USA
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| Abstract: | Polyphenoloxidase (PPO) activity in the glandular trichomes of 32Solanum tuberosum ×S. berthaultii F3 hybrid clones differentially resistant to the green peach aphid,Myzus persicae (Sulzer) was determined. Within this population of segregating clones, aphid infestation levels varied 8-fold, while a 5-fold difference in glandular trichome PPO activity was observed. PPO activity and aphid resistance at three sample dates were correlated as follows: r = 0.38 (P = 0.05), r = 0.31 (P = 0.10), and r = 0.55 (P = 0.01), respectively. The total browning potential test (TBP), a modification of the PPO assay, was developed for use in screening for aphid resistance. The TBP procedure is rapid, inexpensive, and highly effective in identifying aphid resistant clones. |
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