Avian pathogenic <Emphasis Type="Italic">Escherichia coli</Emphasis> bind fibronectin and laminin |
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Authors: | Rosa María Ramírez Yolanda Almanza Rafael González Santos García Norma Heredia |
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Institution: | (1) Departamento de Enfermedades Infecciosas, Unidad Académica de Biología Experimental, Universidad Autónoma de Zacatecas, Calzada de la Revolución Mexicana s/n, Guadalupe Zac, 98600, México;(2) Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás, N.L., 66415, México;(3) Apdo Postal 124-F, San Nicolás, N.L., 66451, México |
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Abstract: | Avian colisepticemia frequently occurs after respiratory tract damage, the primary site for infection allows bacteria to encounter
an exposed basement membrane, where laminin and fibronectin are important components. We investigated the ability of an isolate
of avian pathogenic Escherichia coli to bind fibronectin and laminin. Using Far-western dot blot analysis, we demonstrated the ability of this microorganism to
bind basement membrane proteins fibronectin and laminin. Results from an ELISA-based approach indicate that the binding to
these membrane proteins was bacterial-dose dependent. Furthermore, two specific E. coli polypeptides, of 32 kDa and 130 kDa, reacted with laminin and fibronectin, respectively. Further evaluation of these potential
bacterial adhesins may provide insights into the pathogenesis of colibacillosis. |
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Keywords: | Colisepticemia Escherichia coli Adherence Extracellular matrix Laminin Fibronectin Adhesins |
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