| 农杆菌介导法获得籼稻温敏核雄性不育系转Bt基因植株 |
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| 引用本文： | 马洪亮,杨瑞芳,潘刚,张晓波,黄海清,凃巨民.农杆菌介导法获得籼稻温敏核雄性不育系转Bt基因植株[J].华中农业大学学报,2006,25(5):469-473. |
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| 作者姓名： | 马洪亮 杨瑞芳 潘刚 张晓波 黄海清 凃巨民 |
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| 作者单位： | 浙江大学农业与生物技术学院,杭州,310029 |
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| 基金项目： | 国家高技术研究发展计划(863计划);浙江大学校科研和教改项目 |
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| 摘 要： | 通过农杆菌介导法将Bt融合型基因cry1Ab/cry1Ac导入籼稻温敏核雄性不育系03B198S和03B199S的愈伤组织,经过多轮潮霉素筛选,获得了一批独立转化体,同时,从每一个独立转化体至少再生出5株绿苗。经PCR、RT-PCR检测证实该Bt融合型基因已整合到部分受体基因组中,并表现出不同程度的表达。这些阳性转基因植株在异地异季低温条件下割蔸栽植,部分恢复可育,且表现出很强的抗螟虫性能。
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| 关 键 词： | 籼稻 温敏核雄性不育系 Bt基因 |
| 文章编号： | 1000-2421（2006）05-0469-05 |
| 收稿时间： | 04 2 2006 12:00AM |
| 修稿时间： | 2006-04-02 |
Production of Bt-transgenic Plants Indica Thermo-sensitive Genic Male Sterile (TGMS) Rice Line Using Agrobacterium-mediated Method |
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| MA Hong-liang,YANG Rui-fang,PAN Gang,ZHANG Xiao-bo,HUANG Hai-qing,TU Ju-min.Production of Bt-transgenic Plants Indica Thermo-sensitive Genic Male Sterile (TGMS) Rice Line Using Agrobacterium-mediated Method[J].Journal of Huazhong Agricultural University,2006,25(5):469-473. |
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| Authors: | MA Hong-liang YANG Rui-fang PAN Gang ZHANG Xiao-bo HUANG Hai-qing TU Ju-min |
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| Institution: | College of Agriculture and Biotechnology ,Zhejiang University ,Hangzhou 310029,China |
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| Abstract: | The fused Bt gene cry1Ab/cry1Acwas introducedinto the calli of indica TGMS lines 03B198Sand 03B199Susing Agrobacterirum-mediated method.After multiple-cycle selections withhygromycin,lots of independent transformants were obtained and at least 5 plants were regeneratedfrom each transformant.The PCR and RT-PCR analysis of these transformants confirmed the integration of the fused Bt gene into the genome of some recipient plants and the expression of its mRNA at different levels.Some positive transgenic plants after ratooning turned to befertile at low temperature in the fieldof Hainan at winter,and showedhighlyresistantto leaf folders and stem borers. |
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| Keywords: | indica rice TGMS line Bt gene |
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