水稻抗干尖线虫基因OC-XII的克隆及表达研究

抗性验证试验表明籼稻大白谷抗水稻干尖线虫侵染。为进一步探究此抗性与病程相关蛋白——半胱氨酸蛋白酶抑制剂(Cystatin)的相关性,基于水稻基因组数据克隆籼稻大白谷Cystatin家族的OC-XII基因,分别进行OC-XII、Japonica-OC-XII和Indica-OC-XII蛋白质三维结构构建,并与水稻干尖线虫组织蛋白酶B(Cathepsin B)进行分子对接以验证OC-XII基因编码蛋白对Cathepsin B的抑制功能。通过荧光定量qPCR验证OC-XII基因在线虫侵染前后的表达特性,OC-XII蛋白可以与水稻干尖线虫Cathepsin B蛋白质特异结合,线虫侵染后OC-XII基因在浸染12h~2d期间表达量持续上调,侵染3d后下调。表明OC-XII基因在大白谷抗水稻干尖线虫侵染过程中发挥功能,具有对植物寄生线虫进行生物防治的潜力,有必要对其进行深入研究。

Cloning and Expression of the Gene OC-XII Conferring Resistance to Aphelenchoides besseyi in Rice

The previous resistance verification indicated that Dabaigu (Oryza sativa ssp. indica) resists the infection of Aphelenchoides bessey. To reveal the interaction between the resistance and the pathogenesis-related protein Cystatin, the gene OC-XII was cloned, the docking between OC-XII and A. besseyi Cathepsin B was done, and the expression of OC-XII was tested by quantitative real-time PCR. Results showed that OC-XII bound with Cathepsin B specifily. The expression level of OC-XII was upregulated from 12 hours after infection to 2 days after infection, and downregulated 3 days after infection. These results indicated that OC-XII was involved in the resistance to A. besseyi. The gene has the potential for biological control of plant-parasitic nematodes, and it is necessary to conduct in-depth research.