龙牙百合鳞茎总黄酮提取工艺及抗氧化性研究

目的]探讨龙牙百合鳞茎总黄酮的最佳提取工艺,并研究其抗氧化活性。方法]以龙牙百合鳞茎为研究对象,采用优化超声波法提取总黄酮。在单因素试验的基础上,采用正交试验法研究了乙醇浓度、料液比、提取时间、提取温度对总黄酮提取量的影响,并确定其最佳工艺条件,并对其体外抗氧化活性进行了研究。结果]龙牙百合鳞茎总黄酮的最佳工艺条件如下:提取温度为30℃,乙醇浓度为80%,料液比(g∶m L)为1∶10,提取时间为20 min。在此条件下,百合鳞茎总黄酮提取量为(32.236±0.513)mg/g。抗氧化试验结果表明,龙牙百合鳞茎总黄酮提取液对DPPH·的清除效果优于相同浓度的维生素C溶液,且存在显著差异(P0.05)。结论]该研究可为龙牙百合的综合开发与利用提供理论依据。

Study on the Extraction Technology and Antioxidant Property of Total Flavonoids from Longya lilium Bulb

Objective] To explore the optimum extraction technology of total flavonoids from Longya lilium bulb and study their antioxidant ac-tivity.Method] Taking L.lilium bulb as research object,total flavonoids were extracted from L.lilium bulb by using optimized ultrasonic method. Based on single factor experiment,the effects of ethanol concentration,material-liquid ratio,extraction time and extraction temperature on the ex-traction quantity of total flavonoids from L.lilium bulb were studied and the optimum extraction technology was confirmed by using orthogonal ex-periment.And their antioxidant activities of total flavonoids in vitro were studied.Result] The optimum extraction conditions of total flavonoids from L.lilium bulb were as follows:80% ethanol,solid-liquid ratio of 1:10 (g :mL),extraction temperature of 30 ℃,extraction time of 20 min. Under the optimum conditions,the extraction quantity of total flavonoids was (32.236 ±0.513) mg/g.The results of in vitro antioxidant test showed that the scavenging effects of total flavonoids extract from L.lilium bulb to DPPH? was better than that of vitamin C solution at the same concentration,and there was significant difference(P<0.05).Conclusion] The research can provide theoretical basis for the comprehensive ex-ploration and utilization of L.lilium.