| Isolation, purification and identification of three peptaibols from Trichoderma koningii with antibiotic activity against Ralstonia solancearum |
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| 引用本文： | SHEN Qing-tao CHEN Xiu-lan SUN Cai-yun ZHANG Yu-zhong. Isolation, purification and identification of three peptaibols from Trichoderma koningii with antibiotic activity against Ralstonia solancearum[J]. 浙江大学学报(农业与生命科学版), 2004, 30(4): 439-439 |
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| 作者姓名： | SHEN Qing-tao CHEN Xiu-lan SUN Cai-yun ZHANG Yu-zhong |
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| 作者单位： | StateKeyLaboratoryofMicrobial＇Technology,ShandongUniversity,Ji＇nan250100,China |
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| 摘 要： | The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides,…
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| 关 键 词： | 抗生素 生物防治 隔离 纯化 木霉属 真菌 |
| 文章编号： | 1008-9209(2004)04-0439-01 |
Isolation, purification and identification of three peptaibols from Trichoderma koningii with antibiotic activity against Ralstonia solancearum |
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| SHEN Qing-tao,CHEN Xiu-lan,SUN Cai-yun,ZHANG Yu-zhong. Isolation, purification and identification of three peptaibols from Trichoderma koningii with antibiotic activity against Ralstonia solancearum[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2004, 30(4): 439-439 |
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| Authors: | SHEN Qing-tao CHEN Xiu-lan SUN Cai-yun ZHANG Yu-zhong |
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| Affiliation: | SHEN Qing-tao（State Key Laboratory of Microbial Technology,Shandong University, Ji' nan 250100, China） CHEN Xiu-lan（State Key Laboratory of Microbial Technology,Shandong University, Ji' nan 250100, China） SUN Cai-yun（State Key Laboratory of Microbial Technology,Shandong University, Ji' nan 250100, China） ZHANG Yu-zhong（State Key Laboratory of Microbial Technology,Shandong University, Ji' nan 250100, China） |
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| Abstract: | The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ, Ⅶ and Ⅷ, and their sequences were confirmed with NMR. |
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| Keywords: | biological control peptaibols ESI-MS NMR Trichoderma Ralstonia solancearum |
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