| 小麦抗赤霉病突变体的选育及RAPD分子验证 |
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| 引用本文： | 孙光祖,李忠杰,李希臣,王广金,唐凤兰,张月学,阎文义,孙德全,孙岩.小麦抗赤霉病突变体的选育及RAPD分子验证[J].核农学报,1999,13(4):0-0. |
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| 作者姓名： | 孙光祖 李忠杰 李希臣 王广金 唐凤兰 张月学 阎文义 孙德全 孙岩 |
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| 作者单位： | 黑龙江省农业科学院作物育种研究所,哈尔滨,150086 |
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| 摘 要： | 利用辐射诱变，组织培养和细胞筛选相结合的方法选育出了抗赤霉病突变体９２ｋ８０９该突变体不仅抗赤霉病能力强，且抗性稳定，同时具有优良的农艺性状，比原亲本增产４．９％，经ＲＡＰＤ技术检测，所用的１２８个引物中有１个引物（ＯＰＹ１５）在抗病变体９２ｋ８０９和赤霉病抗原苏麦３号中得到了相同的扩增产物，初步认为该片段与赤霉病的抗性有关。
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| 关 键 词： | 小麦抗赤霉病突变体 RAPD技术 辐射诱变 细胞筛选 组织培养 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
SELECTION OF WHEAT MUTANT WITH RESISTANCE TOFusarium graminearum AND RAPD TECHNIQUE |
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| Sun Guangzu,Li Zhongjie,Li Xichen,Wang Guangjin,Tang Fenglan,Zhang Yuexue,Yan Wenyi,Sun Dequan,Sun Yan.SELECTION OF WHEAT MUTANT WITH RESISTANCE TOFusarium graminearum AND RAPD TECHNIQUE[J].Acta Agriculturae Nucleatae Sinica,1999,13(4):0-0. |
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| Authors: | Sun Guangzu Li Zhongjie Li Xichen Wang Guangjin Tang Fenglan Zhang Yuexue Yan Wenyi Sun Dequan Sun Yan |
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| Abstract: | The wheat mutant 92k809 with resistance to Fusarium graminearum was selected by means of induction mutation of irradiation, tissue culture and cell sreening. The mutant 92k809 has stable resistance to the disease, good agronomical traits with increasing yield of 49% when compared with its parent. The specific DNA fragments, which both 92k809 and resistant material Sumai No.3 own, have been fond by RAPD technique. These fragments, which do not exist in the parent, are related to scab of wheat. |
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| Keywords: | Wheat mutant s resistance to Fusari um grami nearum RAPD radiation induced mutation tissue culture cell screening |
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