| 离子交换层析法提取黏杆菌素的研究 |
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| 引用本文： | 邢维玲,周希贵,王贺祥,宋渊,章红.离子交换层析法提取黏杆菌素的研究[J].中国兽医杂志,2003,39(11):44-46. |
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| 作者姓名： | 邢维玲 周希贵 王贺祥 宋渊 章红 |
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| 作者单位： | 中国农业大学生物学院微生物系,北京,海淀,100094 |
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| 摘 要： | 本文采用D152型弱酸性阳离子交换树脂对多黏类芽孢杆菌(Paenibacillus polymyxa)发酵液中黏杆菌类(Colistin)的提取条件进行了研究。通过静态与动态吸附相结合的方法，确定了影响提取效果的三个重要参数即洗脱液Hcl浓度、上样液浓度和洗脱液流速。结果表明，当上样液浓度为20000IU／ml，1mol／L HCl以2.0ml／min的流速洗脱时可获得最好的提取效果，收率达到52．8％。
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| 关 键 词： | 离子交换层析法 提取技术 黏杆菌素 杀菌作用 饲料利用率 |
| 文章编号： | 0529-6005(2003)11-0044-02 |
| 修稿时间： | 2001年11月1日 |
Study on the process of extracting colistin by ion exchange chromatography |
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| XING Wei-ling,ZHOU Xi-gui,WANG He-xiang,SONG Yuan,ZHANG Hong.Study on the process of extracting colistin by ion exchange chromatography[J].Chinese Journal of Veterinary Medicine,2003,39(11):44-46. |
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| Authors: | XING Wei-ling ZHOU Xi-gui WANG He-xiang SONG Yuan ZHANG Hong |
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| Abstract: | In this paper,the conditions of extracting colistin from the fermentative liquid by using of weak acidic cation exchange resin D152 were studied.Better conditions of extracting,sample consistency is 20 000 U/ml, 1 M HCl as eluant and the elution rate 2 ml/min,were found by the static and dynamic state experiments.Under this condition,colistin yielded at a rate of 52.8%. |
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| Keywords: | colistin extraction ion exchange |
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