胞质雄性不育和核雄性不育辣椒内源激素含量的变化

采用酶联免疫吸附法(ELISA)测定和比较分析了CMS和GMS辣椒不育系(株)和相应保持系(可育株)花蕾和叶片中的内源IAA,Z ZR,GA3,ABA含量及比值的变化。结果表明,CMS雄性不育系和保持系花蕾中IAA,Z ZR,GA3和ABA含量的变化趋势与GMS不育株和可育株间变化趋势基本相同,即不育系(株)IAA,Z ZR,GA3和ABA含量低于保持系(可育株);叶片中Z ZR,GA3和ABA含量变化趋势GMS和CMS也基本一致,即不育系(株)中的含量高于保持系(可育株);花蕾中IAA/ABA,(Z ZR)/ABA,GA3/ABA激素比例的变化GMS与CMS也相同,即不育系(株)比值小于保持系(可育株)。说明虽然GMS和CMS辣椒控制雄性不育的基因不同,但在调节雄性不育时激素的变化规律大多相同,辣椒雄性不育在生理生化机理上可能存在一些相同的特点。

The Contents Changes of Endogenous Hormones in CMS and GMS of Pepper( Capscium annuum L.)

The contents of endogenous hormones in flower buds and leaves,including IAA,Z ZR,GA_3,ABA of two male sterility lines(CMS and GMS) and their corresponding maintainer lines of Pepper(Capscium annuum L)were assayed using ELISA.The experiment also analyzed the ratio of the endogenous hormones.The results as following:The changing tendency of endogenous hormones contents including IAA,Z ZR,GA_3,ABA in the flower bud of CMS and its corresponding maintainer lines consists with the tendency in GMS and its corresponding maintainer lines,that is the contents of IAA,Z ZR,GA_3 and ABA in male sterile lines are lower than in the maintainer lines.There also have the same contents changing tendency of IAA,Z ZR,GA_3 and ABA in the leaves in CMS and GMS,that is the contents of endogenous IAA,Z ZR,GA_3,ABA in male sterile lines are higher than in the maintainer lines.The ratio of endogenous hormones including IAA/ABA,(Z ZR)/ABA,GA_3/ABA in the flower buds has the same changing tendency in CMS and GMS,that is the ratio of male sterile lines is lower than of the maintainer lines.In conclusion,the results indicate that the changes of hormones in the regulating male sterile have the same rules and the male sterile of pepper may have same traits in the mechanism of biochemistry and biophysics,although the dominating gene of the GMS and CMS is different.