半枝莲多糖对小鼠脾淋巴细胞cAMP含量的影响

目的研究半枝莲多糖（sPs）对小鼠脾淋巴细胞环磷腺苷（cAMP）含量的影响。方法60只小鼠随机分为5组，每组12只，即SPS50、100、200mg／（k·d）剂量组，氨茶碱组（阳性对照）和生理盐水组（阴性对照），采用蛋白竞争结合法测定小鼠脾淋巴细胞中cAMP含量。结果SPS低、中、高剂量组的cAMP含量分别为（27．04±6．21）、（31．64±4．57）、（29．95±5．75）pmol×10^7／cells；与生理盐水组比较，中、高剂量组的cAMP含量明显升高，差异有统计学意义（P〈0．01或0．05），低剂量组则无明显变化。结论一定剂量的SPS能升高淋巴细胞cAMP水平，可能是其抑制肿瘤生长，增强小鼠免疫功能的重要途径。

Effect of Scutellaria barbata polysaccharides(SPS) on cAMP level of splenic lymphocytes in mice

Objective To study the effect of Scutellaria barbata polysaccharides （SPS） on cAMP content of splenic lymphocytes in mice. Methods Sixty mice were randomly divided into 5 groups： normal saline （NS） group, aminophylline group, and low （50 mg·kg^-1·d^-1） ,moderate （100 mg·kg^-1 ·d^-1） and high dose （200 mg·kg^-1·d^-1） SPS groups, SPS2 group. Level of cAMP in splenic lymphocytes was detected by competitive protein-binding method. Results Level of cAMP in low, moderate and high dose SPS groups were （27.04 ± 6.21）, （31.64 ± 4.57） and （29.95 ± 5.75） pmol ×10^7/cells, respectively; that of cAMP only in moderate and high dose SPS groups was significantly higher than that in NS group （ P 〈 0.01 or 0.05）. Conclusion SPS is likely to inhibit tumor proliferation and enhance immune function by upregulating cAMP expression of splenic lymphocytes in mice.