应用PCR检测鸡传染性喉气管炎病毒

根据传染性喉气管炎病毒(ILTV)TK基因序列，设计、合成1对引物，应用PCR技术对ILTV以色列疫苗株、河南分离株（ILTV-CG和ILTV-XY）进行PCR扩增，均能扩增出预期大小的目的片段，测序分析和酶切分析证实了PCR产物的特异性，而对其它禽病原体的扩增均为阴性。PCR检测ILTV DNA的最小检测量为21 pg。应用PCR检测人工接种后不同〖JP2〗天数采集的鸡的结膜拭子，接种后第2~5 d均能检测到ILTV。该方法可用于鸡传染性喉气管炎病的诊断和临诊样品检测。

Detection of Infectious Laryngotracheitis Virus with Polymerase Chain Reaction

One pair of primers was designed and synthesized according to the chicken infectious laryngotracheitis virus(ILTV) TK gene nucleotide sequence in the Genbank.When PCR was performed with 2 virulent ILTV,1 vaccine ILTV and other pathogenic microorganisms,the amplified fragment of ILTV was about 1183 bp which could be confirmed by sequencing analysis and restriction endonuclease analysis,others were negative.21 pg ILTV DNA could be detected by PCR.All of the tracheal sponge samples from experimentally infected chickens was amplified a specific 1183 bp fragment of TK gene.The results suggested that the method can be used for detection and diagnosis of ILTV in clinic samples.