Caulogenic callus induction and adventitious bud formation from embryos of long-term stored seeds ofPicea jezoensis |
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Authors: | Shinjiro Ogita Takafumi Kubo Masami Fushitani |
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Affiliation: | (1) Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183 Fuchu, Tokyo, Japan |
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Abstract: | Caulogenic calli with a high differentiation potency were induced from mature embryos ofPicea jezoensis seeds stored over a long time, for 29 years, resulting in the active formation of adventitious buds. Embryos began to induce calli within 3 weeks of cultivating on LP medium containing 3 μM BAP and 1 μM 2,4-D. Then, the calli proliferated and transformed into caulogenic calli with bud primordia in 8 weeks. The caulogenic calli increased actively with the addition of 500 mg/l ofl-glutamine in the medium. Furthermore, caulogenic calli, induced on LP medium containingl-glutamine, resulted in the formation of adventitious buds, which elongated after transferring the calli into LP medium with 0.1 μM BAP, but withoutl-glutamine. It appears that the number of adventitious buds and the process of shoot elongation are influenced by the kind of nitrogen contained in the medium for callus induction. A part of this study was presented at the 107th Annual Meeting of the Japanese Forestry Society (1996). |
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Keywords: | adventitious bud caulogenic callus font-variant:small-caps" >l-glutamine long-term stored seed Picea jezoensis |
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