| 水貂源犬瘟热病毒F基因真核表达载体的构建及鉴定 |
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| 引用本文： | 苏凤艳,全学俊,宗春苗,王卓聪,丁庆东,王全凯.水貂源犬瘟热病毒F基因真核表达载体的构建及鉴定[J].经济动物学报,2007,11(1):23-26. |
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| 作者姓名： | 苏凤艳 全学俊 宗春苗 王卓聪 丁庆东 王全凯 |
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| 作者单位： | 1. 吉林农业大学中药材学院,长春,130118
2. 图门市牧业管理局,延吉,130042 |
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| 摘 要： | 通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。经DNA测序、限制性内切酶分析和PCR鉴定,证实成功地构建了重组质粒,从而为研究犬瘟热新型疫苗奠定了基础。
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| 关 键 词： | 水貂 犬瘟热病毒 F基因 真核表达载体 |
| 文章编号： | 1007-7448(2007)01-0023-04 |
| 修稿时间： | 2006-12-04 |
Construction and Identification of Eukaryotic Expression Plasmid with Fusion Protein Gene of Canine Distemper Virus Isolated from Mink |
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| SU Feng-yan,QUAN Xue-jun,ZONG Chun-miao,WANG Zhuo-cong,DING Qing-dong,WANG Quan-kai.Construction and Identification of Eukaryotic Expression Plasmid with Fusion Protein Gene of Canine Distemper Virus Isolated from Mink[J].Journal of Economic Animal,2007,11(1):23-26. |
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| Authors: | SU Feng-yan QUAN Xue-jun ZONG Chun-miao WANG Zhuo-cong DING Qing-dong WANG Quan-kai |
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| Institution: | 1. Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China; 2. Animal Husbandry Administration Bureau of Tureen, Yanji 130042, China |
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| Abstract: | The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T-A clone technique and was digested with restriction enzyme of Kpn I and BamH I.The F gene was cloned into eukaryotic expression vectorpcDNA3.1.Finally a recombinant plasmid named pcDNA3.1-CDVF was constructed and identified by PCR,restriction enzyme analysis and sequencing. |
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| Keywords: | mink canine distemper virus F gene eukaryotic expression vector |
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