| Al_2O_3-脱乙酰几丁质作为酶固定化载体的研究 |
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| 引用本文： | 邰宁文 吉鑫松. Al_2O_3-脱乙酰几丁质作为酶固定化载体的研究[J]. 蚕业科学, 1997, 23(3): 158-162 |
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| 作者姓名： | 邰宁文 吉鑫松 |
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| 作者单位： | [1]中国农业科学院蚕业研究所 [2]中国科学院上海生物化学研究所 |
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| 摘 要： | Al2O3作核心的脱乙酰几丁质颗粒可用作酶固定化的载体。观察了脱乙酰几丁质的浓度、脱乙酰度和分子量对酶的固定化的影响。发现以浓度为2．0％、脱乙酰度为85．0％、分子量为1．34×106d的脱乙酰几丁质制得的载体用于固定化酶效果最好。所得固定化GOD具有较高的固定化酶活力、热稳定性和工作稳定性。
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| 关 键 词： | Al_2O_3 脱乙酰几丁质 葡萄糖氧化酶 固定化 |
A STUDY ON Al_2O_3-CHITOSAN AS SUPPORTS OF IMMOBILIZED ENZYME |
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| Tai Ningwen, Zhuang Dahuan. A STUDY ON Al_2O_3-CHITOSAN AS SUPPORTS OF IMMOBILIZED ENZYME[J]. Acta Sericologica Sinica, 1997, 23(3): 158-162 |
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| Authors: | Tai Ningwen Zhuang Dahuan |
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| Abstract: | In the present study chitosan-coating Al2O3 was used as supports for enzyme immobilization. It was discovered that different concentrations of Al2O3-chitosan, degrees of deacetylation and molecular weights of chitosan were related to the immobilization. When Al2O3-chitosan was at concentration of 2. 0%, deacetylation at 85% and with a molecular weight of 1 .03 × 106 Dalton, the best immobilization was obtained. The immobilized GOD showed a high enzymatic activity, improved thermal stability and longer operating stability. |
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| Keywords: | Al_2O_3 Chitosan Glucose oxidaes Immobilization |
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