| Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning （HEGS） System |
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| 作者单位： | [1]National Institute of Agrobiological Sciences, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan [2]Present address： School of Environmental Science, University of Shiga Prefecture, Hassaka, Hikone, Shiga 522-8533, Japan |
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| 摘 要： |
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| 关 键 词： | 遗传连锁图谱 基因组扫描 高密度 高效率 系统 快速构建 AFLP标记 籼粳稻杂交 |
| 收稿时间： | 9 June 2009 |
Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning (HEGS) System |
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| Authors: | Akifumi SHIMIZU Shinji KAWASAKI |
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| Institution: | 1. National Institute of Agrobiological Sciences, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan;2. Present address: School of Environmental Science, University of Shiga Prefecture, Hassaka, Hikone, Shiga 522-8533, Japan |
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| Abstract: | High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS (high efficiency genome scanning) is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density (average interval 2.3 cM) map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers. |
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| Keywords: | rice high-density linkage map high efficiency genome scanning system |
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