Morphological and molecular diversity within Algerian cowpea (<Emphasis Type="Italic">Vigna unguiculata</Emphasis> (L.) Walp.) landraces |
| |
Authors: | Naima Ghalmi Marie Malice Jean-Marie Jacquemin Sidi-Mohamed Ounane Leila Mekliche Jean-Pierre Baudoin |
| |
Institution: | 1.Department of Crop Husbandry,National Agronomic Institute,Algiers,Algeria;2.Tropical Crop Husbandry and Horticulture,Gembloux Agricultural University,Gembloux,Belgium;3.Department of Biotechnology, Laboratory of Molecular Biology,Walloon Agricultural Research Centre,Gembloux,Belgium |
| |
Abstract: | Twenty landraces of cowpea (Vigna unguiculata (L.) Walp.) scattered throughout Algeria were compared through morphological and genetic characterization. At the morphological
level, for qualitative characters there was no intra-landrace variation and for quantitative characters the variations were
low except for landrace NAG2 Three different cultigroups were located in Algeria: Biflora that was dominant in the Sahara,
Melanophtalmus in the North and Unguiculata including one landrace in Kabylia and two in Sahara. The AMOVA analysis indicated
that the genetic variation was lower within than among agro-ecological regions. A Mantel test, revealed a correlation between
the qualitative morphological data and the geographical data (R = 0.28; P < 0.01), indicating that the degree of morphological change among landraces was roughly proportional to the geographical
distances separating them. Genetic diversity was analyzed by using 11 random amplified polymorphic DNA (RAPD) and 12 inter-simple
sequence repeat (ISSR) markers. No intra-landrace variability was found. The eleven RAPD primers yielded 77 bands, of which
45 (58.44%) were polymorphic; the genetic similarity ranged from 66.0 to 96.7%. The twelve ISSR primers provided a total of
104 bands, of which 65 (62.5%) were polymorphic; the genetic similarity ranged from 62.8 to 97.8%. cluster analysis showed
a good match between genetic background and geographical distribution, which was confirmed by the results of the Mantel test.
In particular, geographical data and genetic data were found to be correlated: (R = 0.33; P < 0.01) for RAPD, (R = 0.37; P < 0.01) for ISSR, and (R = 0.33; P < 0.01) for a combined RAPD-ISSR dataset. Moreover, despite the absence of significant correlation between morphological
and RAPD data (R = 0.14; P = 0.14), significant correlations between morphological data and both ISSR (R = 0.27, P < 0.05) and a combined RAPD-ISSR dataset (R = 0.22, P < 0.05) were noted. ISSR markers were better linked to morphological variation than were RAPD markers. However, despite this,
genetic distances among these landraces were found to be essentially the same no matter which markers were used. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|