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| 间接ELISA检测禽霍乱抗体方法的建立 | |
| 引用本文： | 黄金海,王英珍,丁伯良,肖西治,李秀丽,赵向华.间接ELISA检测禽霍乱抗体方法的建立[J].动物医学进展,2001,22(2):61-64. |
| 作者姓名： | 黄金海 王英珍 丁伯良 肖西治 李秀丽 赵向华 |
| 作者单位： | 1. Tianjin Institute of Animal Husbandry and Veterinary Medicine, 2. 天津市畜牧兽医研究所, 3. 西北农林科技大学畜牧兽医学院, |
| 基金项目： | 天津市青年科学基金资助项目! 98370 1711 |
| 摘 要： | 用多杀性巴氏杆菌 TJ8菌株制备ELISA抗原 ,与抗鸡 Ig单抗 1 B7酶结合物建立了检测鸡血清抗体水平的间接 ELISA方法。交叉试验、阻断试验、重复性试验等表明该方法重复性好、特异性强 ,灵敏度高。确立了将鸡血清 1 0 0倍稀释监测 ELISA效价(ET)的回归方程 y=1 .4981 + 0 .391 9x(P>0 .0 5 ) ,可用于定量测定。间接凝集试验与ELISA方法的比较试验表明 ,EL ISA法比间接血凝试验敏感性高 4倍以上 |
| 关 键 词： | 鸡 多杀性巴氏杆菌 ELISA 血清抗体检测 |
| 文章编号： | 1007-5038(2001)02-0061-04 |
| 修稿时间： | 2000年12月19 |
The Development of ELISA for Detecting Antibody of Pasteurella Multocida in Chicken |
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| HUANG Jin-hai,WANG Ying-zhen,DING Bo-lian,XIAO Xi-zhi,LI Xiu-li,ZHAO Xiang-hua.The Development of ELISA for Detecting Antibody of Pasteurella Multocida in Chicken[J].Progress In Veterinary Medicine,2001,22(2):61-64. | |
| Authors: | HUANG Jin-hai WANG Ying-zhen DING Bo-lian XIAO Xi-zhi LI Xiu-li ZHAO Xiang-hua |
| Abstract: | The indirect ELISA method for detecting antibody level of Pasteurell amultocida were developed by preparing coating antigen and enzyme conjugate of mouse against light chain of chicken Ig .The method has good repeativity, high speaciality, and sensitivity. A detecting titer of coefficient relation y=1.981+0.3919 ( p> 0.05) was developed under 100 fold dilution sera sample. The ELISA titer is 4 times higher than indirect hemagglutination test (IHT) result. |
| Keywords: | chicken Pasteurell amultocida ELISA |
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