芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析

以耐病性叶用芥菜为材料,克隆和分析了芥菜Rubicso小亚基的基因序列,研究了芜菁花叶病毒（Turnip mosaic virus,TuMV）侵染对Rubicso小亚基基因表达的影响。结果显示：Rubicso小亚基基因全长为842bp,其开放阅读框（ORF）可编码181个氨基酸,其中包括2个α螺旋及4个β折叠二级结构,...

CLONING OF RUBISCO SMALL SUBUNIT GENE FROM MUSTARD AND ANALYSIS OF ITS EXPRESSION IN RESPONSE TO TURNIP MOSAIC VIRUS INFECTION

We cloned the cDNA encoding the Rubisco small subunit of leafy mustard and studied its expression after Turnip mosaic virus (TuMV) infection. The result showed that the full-length cDNA of Rubisco small subunit gene was 842bp and contained an open reading frame (ORF) encoding 181 amino acids, which had two α-helixes, four β-sheets, and some conserved residues. With the accumulation of CP in upper systemic leaves after TuMV infection, mustard growth was impaired. At the same time, expression of Rubisco small subunit was increased before 6 days of inoculation and then gradually decreased, which suggested that the Rubisco small subunit participated in the response of mustard to TuMV infection.