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| 十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析 | |
| 引用本文： | 韩莹琰,张爱红,范双喜,曹家树.十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析[J].核农学报,2011,25(5):916-921. |
| 作者姓名： | 韩莹琰 张爱红 范双喜 曹家树 |
| 作者单位： | 1. 浙江大学蔬菜研究所,浙江杭州310029／北京农学院植物科学技术学院,北京102206 2. 浙江大学蔬菜研究所,浙江杭州,310029 3. 北京农学院植物科学技术学院,北京,102206 |
| 基金项目： | 国家自然科学基金(30871715); 浙江省重大科技项目(2010C12004); 北京市教委科技项目(KM201110020004); 北京市科技新星项目(2010B020) |
| 摘 要： | 为获取C2H2型锌指蛋白基因在物种间的分类和进化关系,分析其在基因结构上的保守性,更好地研究该基因在植物花粉发育中的功能,根据白菜花粉发育相关基因BcMF20 DNA序列设计引物,运用PCR技术分别从十字花科芸薹属和萝卜属18种材料中克隆了C2H2型锌指蛋白基因BcMF20的同源序列。经序列比对分析,BcMF20同源基... |
| 关 键 词： | 十字花科 C2H2锌指蛋白 BcMF20 克隆 系统发育 |
| 收稿时间： | 2010-01-18 |
| 修稿时间： | 2011-02-24 |
CLONING AND EVOLUTIONARY ANALYSIS OF HOMOLOGOUS SEQUENCES OF A NOVEL GENE ENCODING C2H2 ZINC FINGER PROTEIN IN CRUCIFERAE |
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| HAN Ying-yan, ZHANG Ai-hong FAN Shuang-xi CAO Jia-shu.CLONING AND EVOLUTIONARY ANALYSIS OF HOMOLOGOUS SEQUENCES OF A NOVEL GENE ENCODING C2H2 ZINC FINGER PROTEIN IN CRUCIFERAE[J].Acta Agriculturae Nucleatae Sinica,2011,25(5):916-921. | |
| Authors: | HAN Ying-yan ZHANG Ai-hong FAN Shuang-xi CAO Jia-shu |
| Institution: | HAN Ying-yan1,2 ZHANG Ai-hong1 FAN Shuang-xi2 CAO Jia-shu1 (1.Institute of Vegetable Science,Zhejiang University,Hangzhou,Zhejiang 310029,2.Department of Plant Science and Technology,University of Beijing Agricultural,Beijing 102206) |
| Abstract: | In order to elucidate categorization evolvement of C2H2 zinc finger protein,and clarify mechanism on C2H2 zinc finger protein in pollen development among plants at molecular level,the genes encoding C2H2 zinc finger protein analogues from 18 species of genera Brassicaand Raphanusin Cuciferae were obtained by PCR strategy using specific primers designed from the full length of BcMF20,a putative gene encoding C2H2 zinc finger protein which was related to the male sterility.The phylogenetic relationships of th... |
| Keywords: | Cruciferae C2H2 zinc finger protein BcMF20 isolated phylogenic development |
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