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生长调节剂PCPA和2,4-D对番茄果实果糖激酶活性及基因表达的影响
引用本文:史宝中,王卫平,崔娜,董雪飞,李天来. 生长调节剂PCPA和2,4-D对番茄果实果糖激酶活性及基因表达的影响[J]. 核农学报, 2011, 25(5): 1047-1051
作者姓名:史宝中  王卫平  崔娜  董雪飞  李天来
作者单位:1. 沈阳农业大学设施园艺省部共建教育部重点实验室,辽宁沈阳110866/沈阳师范大学实验中心,辽宁沈阳110032
2. 沈阳农业大学生物科学技术学院,辽宁沈阳,110866
3. 沈阳农业大学设施园艺省部共建教育部重点实验室,辽宁沈阳110866/沈阳农业大学生物科学技术学院,辽宁沈阳110866
4. 沈阳农业大学设施园艺省部共建教育部重点实验室,辽宁沈阳,110866
基金项目:国家十二五科技支撑项目(2011BAD12B03); 辽宁省教育厅科学技术研究项目(2008623)
摘    要:研究了花期施用PCPA和2,4-D对发育过程中番茄果实糖含量、蔗糖代谢相关酶和果糖激酶活性及果糖激酶基因表达的影响。结果表明,随着番茄果实的发育,果糖激酶的活性及其基因表达均先上升后下降,成熟时达到最低,但FRK1基因表达量较高;果糖和葡萄糖含量呈递增的趋势,成熟时含量达到最高。PCPA和2,4-D处理后降低了成熟番茄...

关 键 词:番茄  生长调节剂  果糖激酶  基因表达
收稿时间:2011-01-12
修稿时间:2011-04-29

EFFECTS OF EXOGENOUS PCPA AND 2,4-D ON ACTIVITY OF FRUCTOKINASE AND GENE EXPRESSION IN TOMATO FRUITS
SHI Bao-zhong, WANG Wei-ping CUI Na , DONG Xue-fei LI Tian-lai. EFFECTS OF EXOGENOUS PCPA AND 2,4-D ON ACTIVITY OF FRUCTOKINASE AND GENE EXPRESSION IN TOMATO FRUITS[J]. Acta Agriculturae Nucleatae Sinica, 2011, 25(5): 1047-1051
Authors:SHI Bao-zhong   WANG Wei-ping CUI Na    DONG Xue-fei LI Tian-lai
Affiliation:SHI Bao-zhong1,2 WANG Wei-ping3 CUI Na 1,3 DONG Xue-fei3 LI Tian-lai1 (1.Key Laboratory of Protected Horticulture,Ministry of Education,Shenyang Agricultural University,Shenyang,Liaoning 110866,2.Experimental Center,Shenyang Normal University,Liaoning 110032,3.Biological Science and Technology College,Liaoning 110866)
Abstract:Sugar contents,enzyme activities related to sucrose metabolism and fructokinase(FRK),fructokinase gene expression were studied during tomato fruit development after PCPA and 2,4-D treatment,respectively.Results showed that activity and gene expression of fructokinase increased at first then decreased during fruit development in control.Fructokinase activity reached the lowest level,but FRK1 gene expression showed a higher level during fruit ripening stage.However,the contents of fructose and glucose in frui...
Keywords:Solanum lycopersicum  growth regulator  fructokinase  gene expression  
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