Influence of leptin on intestinal function and its protective effect on hepa tic and renal functions after sepsis

AIM: To detect the effect of sepsis on hepatic,renal functions and corresponding enzymes in intestine of mice,and to explore the role of leptin in acute inflammation.METHODS: A mice model of sepsis was made by cecum ligation and perforation,and 96-well spectrophotometry was used to detecte the levels of alanine aminotransferase (ALT),uric acid (UA) and activities of myeloperoxidase (MPO),glutathin-S-transferase (GST),xanthine oxidase (XOD),superoxide dismutase (SOD) in serum and intestinal homogenized fluids,respectively.Hematoxylin-eosin staining was used simultaneously to check the histopathologic changes of intestine.RESULTS: Compared with sham group (330.12 μmol/L±94.15 μmol/L),serum UA level (521.92 μmol/L±91.86 μmol/L) at 6 h after sepsis was significantly higher.12 h after sepsis,both serum ALT (83.55 U/L±40.44 U/L) and UA (474.03 μmol/L±75.22 μmol/L) were significantly higher than those in sham group (66.23 U/L±16.80 U/L and 320.95 μmol/L±99.14 μmol/L,respectively).12 h after leptin injection (0.1 mg/kg,ip) or indomethacin injection (2 mg/kg,ip),the serum ALT and UA levels significantly decreased (vs sepsis group,P<0.05).Moreover,the activities of MPO,GST,XOD and SOD in intestine were changed at different degrees.CONCLUSION: During the process of sepsis,trace dose of leptin injected peritoneally significantly improves and stabilizes the hepatic and renal functions.The mechanisms may be related with those intestinal enzymes associated with metabolism of free radicals,mercapto group and purine.Indomethacin exerts a similar role as leptin though at much higher dose.