Effects of cholecystokinin octapeptide on expression of IL-1β, IL-6, IL-4 and IL-10 in lipopolysaccharide-attacked mice |
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Authors: | NI Zhi-yu CONG Bin LI Shu-jin MA Chun-ling YAN Yu-xian XU Jin-rong ZHANG Guo-zhong |
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Affiliation: | Faculty of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China. E-mail: nzy0603@126.com |
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Abstract: | AIM:To observe the effects of cholecystokinin octapeptide (CCK-8) on the expression of proinflammatory cytokines IL-1β, IL-6 and anti-inflammatory cytokines IL-10, IL-4 in LPS- attacked mice. METHODS:Kunming mice were randomly assigned and injected intraperitoneally with LPS alone or/and CCK-8 at different time points. The expression of IL-1β, IL-6, IL-10 and IL-4 in the serum and lung tissues were assayed by ELISA and RT-PCR. RESULTS:The expression of IL-1β, IL-6, IL-10 and IL-4 were upregulated in LPS-attacked mice. Pre-treatment of CCK-8 decreased both IL-1β and IL-6 expression and augmented IL-10 and IL-4 expression in LPS-attacked mice. CONCLUSIONS:CCK-8 exerts an anti-inflammatory effect by inhibiting the expression of IL-1β, IL-6 and increasing the expression of IL-10, IL-4 in LPS-attacked mice, which could alleviate the inflammatory response in lung tissue. |
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Keywords: | Cholecystokinin Lipopolysaccharides Interleukins |
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