Effects of angiotensin Ⅱ on ATP binding cassette transporter A1 in THP-1 derived foam cells |
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Authors: | CHEN Zhi-jian WANG Yan-fu LIAO Yu-hua MEI Chun-li PENG Hong-yu GUO He-ping |
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Affiliation: | Cardiovascular Department, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China. E-mail: drchenok@sohu.com |
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Abstract: | AIM:To study the influence of angiotensin Ⅱ (AngⅡ) on ATP-binding cassette transporter A1 in THP-1 derived foam cells. The variance of the expression of ABCA1, the content and the effluent rate of cholesterol were also investigated. METHODS:The regulatory effect of AngⅡ on the expression of ABCA1 mRNA and protein in THP-1 derived form cells were measured by RT-PCR and Western blotting. The effect of variance of cholesterol content was measured by zymochemistry via-fluorospectrophotometer, cholesterol effluent was measured by liquid scintillator. RESULTS:A positive facilitative effect of Ang Ⅱon form cells was observed. Total cholesterol content were increased significantly by Ang Ⅱ treatment (P<0.05). The mRNA and protein of ABCA1 were down-regulated significantly by Ang Ⅱ stimulation (P<0.05). Irbesartan reduced the total cholesterol content significantly (P<0.05). Meanwhile, the increase in the effluent rate of cholesterol and the expression of ABCA1 were observed (P<0.05). CONCLUSION:The effects of Ang Ⅱ on the formation of foam cells and atherosclerosis may be correlated to the activation of AT1 receptor and down-regulation of ABCA1. |
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Keywords: | Angiotensin II ATP-binding cassette transporters Arteriosclerosis Foam cells |
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