Selenium-enriched Spirulina platensis promotes proliferation of hepatocytes in rat partial hepatectomy |
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Authors: | HUANG Feng HUANG Zhi ZHENG Wen-jie YANG Fang DING Zi-hai |
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Institution: | 1.Medical School, Foshan Science and Technology College, Foshan 528000, China;2.College of Life Science and Technology, Jinan University, Guangzhou 510632, China;3.Anatomical Institute of Minimally Invasive Surgery, South Medical University, Guangzhou 510470, China. E-mail: thzwj@jnu.edu.cn |
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Abstract: | AIM: To explore the effects of selenium-enriched Spirulina platensis (Se-SP) on proliferation of hepatocytes in rat hepatectomy. METHODS: Rat hepaectomy model was conducted using male Wistar rats. The rats were randomized into five groups: operation groups with 150 (H), 50 (M) and 15 (L) mg·kg-1·d-1 of Se-SP, placebo-control (P) and sham operation group (F). Activities of glutathione peroxidase (GPx) and thioredoxin reductases (TR) in hepatocytes were determined by chemical colorimetry. The expression index of proliferating cell nuclear antigen (PCNA) in hepatocytes was detected by immunohistochemistry, and the level of [3H]-TDR incorporation in regenerative hepatocytes was analyzed by radio-immunity. RESULTS: Activity of GPx and TR, PCNA expression index as well as [3H]-TDR insertion in hepatocytes (in vitro) were obviously higher (P<0.05) in L groups than those in P and F groups. All parameters were significant changed (P<0.05) after operation in H, M, L and P groups whereas some slightly change in F group. Furthermore, correlation analysis showed that the levels of GPx and TR in hepatocytes all showed positive correlation with PCNA expression (r2=0.77 and 0.87, respectively) and with [3H]-TDR incorporation level (r2 = 0.73 and 0.84, respectively) in hepatocytes. CONCLUSION: Se-SP enhances hepatocyte proliferation in rat hepatectomy, up-regulation of selenoenzymes might be responsible for this effect. |
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Keywords: | Spirulina platensis Selenium Hepatectomy Proliferating cell nuclear antigen |
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