Effects of aging and hypoxia on morphology of cultured rat pulmonary arterial smooth muscle cells |
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| Authors: | SHEN Dan-tong FU Xiang-yang HUANG Qiao-bing |
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| Institution: | 1.Geriatric 4th Department, The General Hospital of Guangzhou Military Region PLA, Guangzhou 510010, China;2.Department of Cardiology, Zhujiang Hospital, Nanfang Medical University, Guangzhou 510282, China;3.Key Laboratory for Shock and Microcirculation, Nanfang Medical University, Guangzhou 510515, China. E-mail: shendantong@sina.com.cn |
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| Abstract: | AIM:To observe the effects of aging and hypoxia on morphology of cultured rat pulmonary arterial smooth muscle cells (PASMCs). METHODS:The cells were divided into four groups: young and normoxic group (A group), aging and normoxic group (B group), young and hypoxia group (C group), aging and hypoxia group (D group). Afterwards, the different morphological variation was observed by means of optical microscope, immune histochemistry and immune fluorescence. RESULTS:Huge differences in morphological characters in PASMCs in hypoxia and in normoxic were observed, particularly, the difference was clearly shown in F-actin concentration and array in the cytolymph. Compared with normoxic group, the concentration of SM-α-actin in hypoxic PASMCs group decreased sharply. CONCLUSION:Aging and hypoxia lead to morphological change in PASMCs. Both factors stimulate the phenotypic modulation in PASMCs, but the phenotypic modulation effect is more apparent in the condition of hypoxia. |
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| Keywords: | Smooth muscle cells Aging Anoxia SM-α-actin |
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