Effect of smoking on GSH and γ-GCS in lungs of rats and the protective role of N-acetylcysteine |
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Authors: | ZOU Zhao-xia HE Zhi-yi RAN Pi-xin |
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Affiliation: | The First Affiliated Hospital, Guangzhou Medical College, Guangzhou Institute of Respiratory Disease, Guangzhou 510182, China. E-mail:pxran@vip.163.com |
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Abstract: | AIM: To investigate the effects of cigarette smoking on the GSH and γ-GCS in SD rats, and the protective role of N-acetylcysteine (NAC). METHODS: SD rats were exposed to cigarette smoke, and GSH concentrations in BALF were measured by GSH-specific DTNB-GSH reductase recycling assay. Expression of γ-GCS protein in lung tissue was determined by immunohistochemistry. RESULTS: Exposure to cigarette smoking increased GSH content in BALF and the expression of γ-GCS protein in the lung tissue of SD rats. CONCLUSION: Cigarette smoking affects intracellular GSH concentration and the expression of γ-GCS protein in lungs of SD rats. NAC attenuates the inflammatory reaction resulting from cigarette smoking. |
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Keywords: | Smoking Rats Glutathione Glutamylcysteine synthetase |
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