Effects of high-glucose on proliferation and apoptosis in endothelial progenitor cells of type 2 diabetes mellitus |
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Authors: | WANG Hong-xiang ZHAO Shi LI Bin-gong MAO Hong SHAO Shi-ying |
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Affiliation: | 1.Department of Hematology,Central Hospital of Wuhan City,Wuhan 430014,China;2.Cardiology Department;3.Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
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Abstract: | AIM: This study aimed to observe the effects of high-glucose on proliferation and apoptosis of endothelial progenitor cells (EPCs) in type 2 diabetes mellitus patients,and tried to elucidate their possible role.METHODS: Various concentrations of glucose were added to the culture system of EPCs from 25 cases of type 2 diabetes mellitus patients (DM group) and 25 cases of healthy volunteers (control group).MTT assays were used to detect the proliferative rates.Annexin-V/PI stains were used to detect the apoptotic rates,and RT-PCR to detect the expression level of bcl-2 and bax.RESULTS: Proliferative activity of EPCs in both control group and DM group were attenuated when concentration of glucose was 33 mmol/L,while apoptotic rates increased.No significant change of proliferative rate and apoptotic rate of EPCs in DM group and control group in the presence of 5 mmol/L glucose was observed.The expression level of bax of EPCs in both DM group and control group increased while expression level of bcl-2 did not change much in the presence of 33 mmol/L glucose.CONCLUSION: High-glucose attenuates proliferative activity of EPCs and increases the apoptotic rate.Upregulation of bax may be its possible role. |
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Keywords: | Glucose Endothelial progenitor cells Diabetes mellitus Cell proliferation Apoptosis |
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