Effect of inhaled nitric oxide on aquaporin expression in newborn rat lung in acute hyperoxic lung injury |
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Authors: | JIANG Jing-jing WU Xi-ling DU Jing DU Li-zhong |
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Institution: | Children’s Hospital, Zhejiang University, Hangzhou 310003, China. E-mail: dulizhong@yahoo.com.cn |
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Abstract: | AIM:To investigate the effect of inhaled nitric oxide on aquaporin expression and alveolar epithelial fluid transport in newborn rats with acute hyperoxic lung injury. METHODS:32 newborn SD rats were randomized to breathe for 48 h room air (C), >95%O2 (O), >95%O2+10-5 NO (NO only in the first 24 h, ONO), room air + NO (CN). Then, the rats were killed, the lung wet-to-dry weight ratio (QW/QD), the histology, and AQP1, AQP5, α1-NKA, α-ENaC mRNA expressions in the lungs were measured. RESULTS:Compared with C group, the QW/QD in O group significantly increased (P<0.01), and AQP1 mRNA expression decreased significantly (P<0.01). Compared with O group, ONO group had a lower level of QW/QD (P<0.05), and AQP1 mRNA expression increased (P<0.05). AQP5 mRNA expression in all groups remained unchanged. CONCLUSION:In newborn rats with acute hyperoxic lung injury, inhaled 10-5 nitric oxide for 24 h may attenuate lung edema and increase AQP1 mRNA expression, suggesting that inhaled 10-5 nitric oxide for 24 h may promote the AQP1 expression in lung in this model of acute lung injury. |
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Keywords: | Nitric oxide Acute lung injury Aquaporins |
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