Ultrasound promotes the delivery of an antisense oligo-peptide nucleic acid targeting against PDGF-B mRNA to inhibit the vascular restenosis

AIM:To elucidate the effect of antisense oligo-peptide nucleic acid (PNA) against PDGF-B mRNA on in vivo proliferation of VSMCs and vascular restenosis. METHODS:A rabbit vascular restenotic model was constructed and a synthesized PNA was transfected into the vascular cells using a therapeutic ultrasound for the gene drug delivery. The proliferation of intimal VSMCs was observed by monitoring the expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor B chain mRNA (PDGF-B mRNA) with the LSAB and in situ hybridization, respectively. The intimal thickness and area were measured. RESULTS:PNA delivered by therapeutic ultrasound significantly inhibited the expression of PCNA and PDGF-B mRNA by intimal VSMCs 1 week after denudation with the inhibitory rates of 84.19% and 64.95%, respectively and reduced the intimal thickness and area. CONCLUSION:The PNA against PDGF-B mRNA delivered by therapeatic ultrasound inhibits the proliferation of rabbit VSMCs and the formation of intima.