Trichosanthin-induced apoptosis in melanoma B-16 cell line |
| |
Authors: | ZHOU Xin-yang ZHANG Tian-yi GU Jun-yi DING fei |
| |
Institution: | Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China.E-mail: prozxy@ntu.edu.cn |
| |
Abstract: | AIM: To investigate the antitumor effect and mechanism of trichosanthin (TCS) on melanoma B-16 cells.METHODS: (1) The injury of B-16 cells by trichosanthin was observed with SCGE and hoechst33258 staining method.(2) LCSM and specificity fluorescent probe Fluo-3/AM,H2DCF-DA,DAF-FM diacetate were applied to analyze the dynamic changes of Ca2+,ROS and NO in single cell cultured with TCS.Simultaneously,the relationship between ROS,NO and increase of Ca2+ was also revealed.RESULTS: (1)When treated with TCS (50 mg/L) for 3 h and 6 h,neither cytotoxicity assay nor SCGE showed the differences compared with control group.After 12 h incubation,specificity phenomena of DNA injury-comet tail appeared in SCGE and chromatin condensation even apoptotic body formation were seen by Hoechst33258 staining.(2) TCS (50 mg/L) evoked rapid enhancement of the production of Ca2+,ROS and NO in the cell and the differences between TCS and control group had statistical significance (P<0.01).Augment of ROS and NO was involved in the increase of Ca2+.CONCLUSION: Trichosanthin has relatively strong cytotoxicity on melanoma B-16 cells and contributes to apoptosis as well.Trichosanthin-induced apoptosis is related to the increase of Ca2+,NO and ROS in the cells. |
| |
Keywords: | Trichosanthin Melanoma B-16 cells Calcium Reactive oxygen species Nitric oxide |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|