Study on Application of Candida utilis CU-3 in Cotton Straw Feed Fermentation

This experiment was conducted to study the effects of cotton straw fermentated by Candida utilis CU-3,in order to obtain a compound preparation with high efficiency to improve the quality of cotton straw feed.The gossypol tolerance of the strain was tested through the plate tolerance with gossypol acetate as the sole carbon source,and the optimal detoxification conditions of CU-3 were optimized by the solid fermentation.CU-3 mixed fermentation with other preparations were applied to the realistic production,the chemical substances such as crude protein,crude fiber and crude fat in the cotton straw were detected to verify the fermentation effect.The results showed that CU-3 could be grown in 1 000 mg/kg gossypol acetate medium,which was the sole carbon source.The increase of fermentation time and inoculation amount,the detoxification rate of CU-3 on cotton straw increased significantly (P<0.05),and the detoxification rate was the best when the water content was 60% in single-factor cotton straw solid fermentation.The variance analysis of response surface model showed that the equation regression was significant (P<0.01),the maximum degradation rate was 70%,when the conditions were 60% water content of cotton straw,10% inoculation amount and 30 days fermentation time.CU-3 mixed with other preparations,the sensory evaluation scores were all excellent,cotton straw had golden color,clear structure and mellow smell.The free gossypol content of cotton straw was significantly reduced (P<0.05),the minimum content of free gossypol was 62.00 mg/kg.The crude protein content was significantly increased (P<0.05),the highest reached 9.41%.The crude fat content was up to 14.50 g/kg.The crude fiber content was significantly reduced (P<0.05).The cadmium,lead and chromium contents of each treatment group reached the standard.The best compound preparation was CU-3 with 2% corn flour.The application effect tests were carried out in the three farms of 142 Regiment,Manas and Beiwucha.The pH of the cotton straw were between 5.05 and 5.20 after fermentation,the detoxification rates were all above 70% and the highest reached 78.5%,the crude protein contents were 7.97%,8.24% and 9.89%,the crude fiber content were 35.4%,34.5% and 31.2%,respectively,the crude fat content was up to 10%.In this experiment,CU-3 and its compound preparation could effectively improve the detoxification efficiency and feed quality of cotton straw under optimized fermentation conditions,and provide experimental basis for the application of cotton straw feed in Xinjiang.