The expression of NF-κB and ICAM-1 in rat brain of experimental intracerebral hemorrhage and cerebral microvascular endothelial cells injured by hydrogen peroxide |
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Authors: | ZHI Yi-hui LI Xing-qun LUO Yun WAN Sai-ying LI Tong |
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Institution: | 1.ICU, Zhejiang TCM Hospital, Hangzhou 310000, China;2.Institution of TCM and Western Combined Medicine, Xiangya Hospital, Center South University, Changsha 410008, China;3.The First Affiliated Hospital, Zhejiang Medical College, Hangzhou 310000, China |
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Abstract: | AIM: To discuss the possible mechanism of the inflammation after intracerebral hemorrhage (ICH) and the relationship of nuclear factor-kappa B (NF-κB) and intercellular adhesion molecule-1 (ICAM-1). METHODS: The expression of NF-κB and ICAM-1 were detected by immunohistochemistry, in situ hybridization, immunocytochemistry and Western blotting techniques in rat brain of experimental ICH and cerebral microvascular endothelial cells (RCMECs) injured by hydrogen peroxide. RESULTS: The expression of NF-κB p65 and ICAM-1 were up-regulated in rat brain after ICH. The ICAM-1 reached the peak at 1 day while the NF-κB at 4th day. NF-κB p65 expressed remarkably in cultured RCMECs immediately after injured by hydrogen peroxide, while ICAM-1 expressed remarkably 2 hours later. PDTC, an inhibitor of NF-κB, down-regulated the expression of NF-κB and ICAM-1. CONCLUSION: NF-κB induces the expression of ICAM-1 in RCMECs injured by reactive oxygen species (ROS). |
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Keywords: | Cerebral hemorrhage Intercellular adhesion molecule-1 NF-kappa B |
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