Effect of VEGF on proliferation and differentiation of neural stem cells from the hippocampal gyrus of rat in vitro |
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Authors: | GUO Ying SHI De-jin LIANG Chao-feng WANG Hui LI Wen-sheng YE Zhuo-peng HU Li-ping LI Yan |
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Affiliation: | 1.Department of Neurosurgery,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China;2.Department of Histology & Embryology,Basic Medical College,Sun Yat-sen University,Guangzhou 510080,China.E-mail:sumsgy@21cn.com |
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Abstract: | AIM: To observe the effects of vascular endothelial growth factor (VEGF) on the proliferation and differentiation of neural stem cells (NSCs) of rats in vitro.METHODS: NSCs isolated from the hippocampal gyrus of SD rats were primary cultured and subcultured,and then divided into two groups: (1) the cells in VEGF group were treated with 150 μg/L VEGF in the culture system,and VEGF was removed at the 7 th day;(2) control group (without VEGF treatment).The cellular morphology of two groups was observed by contrast phase microscope.Nestin and NF-200 expressing cells were detected via immunofluorescence method.The percentages of the immunostaining positive cells in each group at the 7 th day and at the 11 th day were determined.RESULTS: At the 7 th day,the percentage of nestin positive cells in VEGF group was 52.19%±7.95%,vs 29.26%±4.12% in control group (P<0.01).The percentage of NF positive cells in VEGF group was 22.33%±4.13%,vs 38.62%±5.31% in control group (P<0.01).At the 3 th day after VEGF was removed,the percentage of NF positive cells in VEGF group was 43.10%±3.70%,vs 30.56%±4.16% in control group (P<0.01).CONCLUSION: VEGF stimulates the proliferation of neural stem cells and inhibits their differentiation. |
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Keywords: | Neural stem cells Vascular endothelial growth factor Hippocampus |
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