Effects of Gax gene transfection on proto-oncogene expression and proliferation of PASMCs in rats under hypoxia conditions |
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Authors: | XIA Shi-jin HU Ming-dong BAI Li QIAN Gui-sheng |
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Affiliation: | Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China.E-mail: qiangs@mail.tmmu.com.cn |
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Abstract: | AIM: To explore the effects of Gax gene transfection on expressions of c-fos and c-jun mRNA and proliferation of pulmonary arterial smooth muscle cells (PASMCs) in rat under hypoxia.METHODS: PASMCs were transfected with Gax gene by Ad-Gax.Under normal oxygentention (21% O2) or hypoxia (2.5% O2) for 12 h condition,expressions of Gax mRNA and protein in PASMCs were detected by RT-PCR and immunocytochemistry.The expressions of c-fos and c-jun mRNA were evaluated by RT-PCR.[3H]-TdR incorporation was used to measure the PASMCs proliferation.RESULTS: The Gax overexpression in transfection group was confirmed by RT-PCR and immunocytochemistry.Under normal oxygentention or hypoxia,the c-fos and c-jun mRNA levels in transfection group were lower than those in the non-transfection group,respectively (P<0.05).[3H]-TdR incorporation in the transfection group was lower than that in non-transfection group (P<0.05,P<0.01).CONCLUSION: Gax overexpression might inhibit the PASMCs proliferation induced by hypoxia through downregulating the expressions of c-fos and c-jun. |
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Keywords: | Genes Gax Anoxia Proto-oncogenes Pulmonary arterial smooth muscle cells Cell proliferation |
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