Expression and Bioinformatic Analysis of ADIPOQ Gene in Hanper Sheep |
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Authors: | LIU Aiju ZHAO Juan ZHANG Xin ZHOU Rongyan TIAN Shujun BAI Ying CHEN Xiaoyong |
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Affiliation: | 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;2. College of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China |
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Abstract: | The aim of this study was to investigate the expression and biological characteristics of adiponectin (ADIPOQ) gene in longissimus dorsi muscle of Hanper sheep at different months of age.The ADIPOQ gene mRNA expression was detected in longissimus dorsi muscle of Hanper sheep at 3 different growth periods (1,7 and 13 months old) by Real-time quantitative PCR.The bioinformatics method was used to analyze the nucleotide and amino acid sequences of ADIPOQ gene,protein characteristics and network interaction protein.The results showed that ADIPOQ gene was expressed in longissimus dorsi muscle of Hanper sheep,which showed an increasing trend with the growth of the months age without significant difference (P>0.05).There was higher homology of ADIPOQ gene with Capra hircus (98.60%,99.00%) and Bos taurus (98.60%,87.00%) in nucleotide and amino acid sequences.The results of physical and chemical properties analysis showed that the molecular formula of ADIPOQ protein was C1172H1776N314O349S5,the molecular mass was 26.00 ku,and the theoretical isoelectric point (pI) was 5.88,which suggested that the protein was acidic.The average value of ADIPOQ hydrophilic protein (GRAVY) was -0.403,which indicated that the protein was hydrophilic and didn’t belong to transmembrane protein.In addition,the signal peptide sequence was Met1-Ala17,and the cleavage site was between Ala17 and Arg18.ADIPOQ existed collagen structure domain and C1Q conserved structure domain at position 45-101 and 101-237,respectively.The secondary structure mainly composed of random coil (66.95%).The construction of protein interaction network indicated that ADIPOQ might interact with ADIPOR1,ADIPOR2,CDH13 and LEP.The results provided a theoretical basis for further exploring the molecular biological functions of ADIPOQ gene in the process of muscle development in Hanper sheep. |
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Keywords: | Hanper sheep ADIPOQ gene expression bioinformatics |
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