Distribution and identification of immunocytes in humanized SCID mouse model |
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Authors: | LIN Chen TAN Yu-bo BAI Xue CHEN Shao-hua YANG Li-jian JIANG Zhen-you LI Yang-qiu |
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Institution: | 1.Department of Microbiology and Immunology, 2 Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China. E-mail: tlinc@jnu.edu.cn |
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Abstract: | AIM: Humanized-NOD/SCID(hu-NOD/SCID) mouse model was established and the level of immune reconstitution was assessed in this model. METHODS: Mononuclear cells (MNC) and CD34+ cells were isolated or sorted from cord blood(CB). Human CD45, CD19, CD3 markers on cells from NOD/SCID murine peripheral blood(PB), bone marrow(BM), thymus were detected by FCM from 4 to 10 weeks after hematopoietic stem cell transplantation. After 10 weeks, the gene expressions of the human β2M and RAG2 were detected by RT-PCR in PB or bone marrow of mice model. RESULTS: Human CD45, CD19, CD3 cells populations in PB and BM were found by flow cytometry in mice model transplanted with CD34+ cells or CB MNC from 4 to 10 weeks. The highest positivity of human lymphocytes was at 8 week after transplantation. The levels of human cell engraftment in mice transplanted with CD34+ cells were higher than those in mice transplanted with CB MNC. The mRNA of human β2M and RAG2 were found by RT-PCR in BM.CONCLUSION: The higher level of human lymphocyte engraftment is established in NOD/SCID mouse model transplanted with CD34+ compared with CB MNC. The maturation of T lymphocytes could be happened in bone marrow of mice model. |
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Keywords: | Hematopoietic stem cells NOD/SCID mice CD34+ cells T-lymphocytes |
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